Figure 4
From: Comparative study reveals better far-red fluorescent protein for whole body imaging
Imaging far-red and near-infrared fluorescent proteins in cells.
(a) Fluorescence intensity of transiently transfected HEK293T cells expressing Katushka2S, mCardinal, or mNeptune2.5 was measured over time using listed excitation and emission settings on an IVIS Lumina system. Graphs show mean values ± SEM when larger than the symbol for fluorescence intensity at each time point. Fluorescence from each protein was normalized to background fluorescence from untransfected cells. (b) HEK293T cells transiently transfected with listed fluorescent proteins were imaged and fluorescence radiance intensity quantified on an IVIS Spectrum 48 hours after transfection. Contour plots show data for various pairs of excitation and emission filters based on the depicted pseudocolor scale.
