Figure 2
From: miR-19b downregulates intestinal SOCS3 to reduce intestinal inflammation in Crohn’s disease
The SOCS3 3’-UTR contains conserved miR-19b target sites. (a), The fold-changes of eight miRNAs predicted by three algorithms in CD intestinal tissues compared to controls, as analysed by qRT-PCR (n = 20 per group; *p = 0.012, miR-19b group;***p = 0.000395, miR-148a group). (b), Schematic illustration of conserved duplexes formed by SOCS3 3’UTR and miR-19b interactions. The predicted free energy of the hybrid is noted. The predicted structure of the base-paired hybrid is shown. Paired bases are marked by a black line. Sequence alignment of the putative miR-19b binding sites across species is shown. The complementary seed sites are marked in red and all nucleotides in these regions are conserved among several species, including human, mouse and rat.
