Figure 7

The relationship between miR-19b and SOCS3 in the pathogenesis of colitis(a), Quantitative real-time PCR analysis of miR-19b levels in different treated groups. (n = 8 mice per group;^*p = 0.021030, control group vs. TNBS group;^***p = 0.000628, TNBS group vs. PEI+pre-miR19b group; ^*p = 0.022823, PEI group vs. PEI+pre-miR-19b group;^***p = 0.000837, PEI+pre-scramble group vs. PEI+pre-miR-19b group). (b), The Western blot analysis of colon sections for different treated mice implanted with SOCS3 antibody is presented. Upper panel: representative image; lower panel: quantitative analysis from three independent experiments. The original whole blot pictures are available in Supplementary Fig. S7. (n = 8 mice per group;^***p = 0.0000, normal control vs. PEI+pre-miR19b group;^***p = 0.000015, TNBS group vs. PEI+pre-miR19b group;^***p = 0.000010, PEI+pre-scramble group vs. PEI+pre-miR-19b group; ^***p = 0.0000, PEI group vs. PEI+pre-miR-19b group). (c), SOCS3 mRNA was normalized to GAPDH expression in colon tissues of treated mice by PCR. None significance were detected in all groups. (d), Tissue homogenates from each group mice were subjected to ELISA to quantify MIP-3α protein levels. Results are expressed as ng of MIP-3α per mg of total homogenate protein (n = 8 mice per group;^**p = 0.002473, control group vs. TNBS group;^***p = 0.000077, TNBS group vs. PEI+pre-miR19b group;^***p = 0.000015, PEI group vs. PEI+pre-miR-19b group;^***p = 0.00013, PEI+pre-scramble group vs. PEI+pre-miR-19b group).