Figure 3
Blockade of LT/LIGHT signaling inhibits viral replication in nervous tissue.
(a) Rag1–/– mice (n = 4 to 7/group) were infected with 2 × 106 pfu of HSV-1 and treated with either LTβR-Ig or control protein on day -1 and day 5 p.i.. At the indicated time points of figures, mice were euthanized. Footpad, DRG (L3, L4 and L5) and spinal cord were collected. Viral loads in different tissue homogenates were determined by plaque assay. (b) Rag1–/– mice (n = 4 /group) were infected with 2 × 106 pfu of HSV-1 via footpad injection. For group with sciatic nerve (SN) resection, one segment of the SN was removed on day 2 (d2) p.i., For the group with SN resection and blockade of LT/LIGHT, 100 ug/mice of LTβR-Ig was administrated on day 2 p.i. (after sciatic nerve resection) and day 8 p.i. Data are representative of two independent experiments. Statistical analysis for a. unpaired t test, Error bar represents SEM, *p < 0.05, **p < 0.01, ***p < 0.001; ns, no significant difference. b. log rank test.
