Figure 2

Effect of curcumin on apoptosis- and autophagy-related protein expression in prostate cancer cells.
(A): Prostate cancer cell lines were incubated in curcumin and harvested at various time points. After treatment with 50 μM curcumin for the indicated intervals, LNCaP, DU145, PC-3 and PC-3M cells were lysed and immunoblotted using antibodies against PARP, Beclin-1, LC3 and β–actin. (B): The time course of the protein expression of Beclin-1, p62 and LC3 following treatment with 50 μM curcumin for the indicated intervals based on western blot. β–actin was used as a loading control.