Figure 7
Curcumin suppresses tumor growth via oxidative-stress-induced vacuolation-mediated cell death in a PC-3M cell-based orthotopic tumor model.
(A): Xenogen IVIS spectrum bioluminescence imaging of orthotopic prostate tumor growth. SCID mice were injected with PC-3M cells into the prostate and treated with curcumin (1.5 mg/mouse, intraperitoneal), curcumin and NAC (1 g/kg, oral), vehicle (control) or NAC alone. The mice were then subjected to Xenogen imaging at the indicated time points. (B): Quantitative analysis of the Xenogen imaging signal intensity (photons/sec/cm2/sr) over time after cell injection. (C): Representative imaging results from the indicated treatment protocols at 35 d after cancer cell injection are shown. (D): Relative weights of the tumors isolated from each group of tumor-bearing mice at the end of the experiment. (E and F): The tumor tissues were examined via immunohistochemical staining using the anti-Ki-67 antibody (E) or hematoxylin and eosin staining (left panel: magnification of the boxed areas in the vehicle- and curcumin-treated groups) (F). (G): The expression of ubiquitinated proteins in orthotopic tumors. Equal protein loading was confirmed in the western blot by probing for β-actin.
