Figure 2 | Scientific Reports

Figure 2

From: Epigenetic regulation of the nuclear-coded GCAT and SHMT2 genes confers human age-associated mitochondrial respiration defects

Figure 2

Effects of reprogramming of fibroblasts on age-associated mitochondrial respiration defects. (a) Immunostaining of original fibroblasts and fibroblasts redifferentiated from hiPSCs (reprogrammed fibroblasts) with antibody to a fibroblast-specific marker enzyme, namely the beta subunit of prolyl 4-hydroxylase. R3S, R121, R107 and R102 represent fibroblasts reprogrammed from the original fibroblasts TIG3S, TIG121, TIG107 and TIG102, respectively. Bars, 100 μm. (b) Estimation of O2 consumption rates of original and reprogrammed fibroblasts. Black and open bars are original fibroblasts from young and elderly subjects, respectively. Gray bars represent reprogrammed fibroblasts. Experiments were performed in triplicate; error bars indicate ± SD. *P < 0.05.

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