Figure 1

Micrographs of BOTRYCO-2 associated with B. braunii.
(a) A differential interference contrast image of B. braunii Ba10−/BOTRYCO−2 showing the association of B. braunii and BOTRYCO-2. Alcian blue was used to stain the acidic polysaccharides. Arrowheads indicate the outer most rim of the extracellular matrix (EM). Arrows indicate BOTRYCO-2 associated with the EM. (b) CARD-FISH of B. braunii Ba10−/BOTRYCO−2 with the BOTRYCO-2-specific probe BAG645 (yellow-green). Algal cells are indicated in orange because of chlorophyll autofluorescence.