Figure 3
A 16S rDNA NJ tree of Alphaproteobacteria focusing on the phylogenetic location of BOTRYCO-2.
Numbers in parentheses indicate the GenBank accession numbers. The tree was rooted with Rickettsia typhi (L36221). T indicates the type strain of the bacterial species. Bootstrap values (>60%) on the basis of NJ and ML are shown above and below the branches, respectively, whereas the values for branches that are not consistent between the NJ and ML trees are not shown. Branches obtaining 100% values for both NJ and ML are indicated in bold. Well-defined clades that are distantly related to BOTRYCO-2 are compressed. A full version of the NJ tree including all OTUs is provided in Supplementary Fig. S10. The target of the BOTRYCO-2-specific probe and primer is indicated. Note that the corresponding region within EU703181 (indicated by the gray arrow) is 1 bp different from the probe sequence, so that EU703181 may be detected by FISH and PCR under less stringent conditions.
