Figure 4

The merged fluorescent image (DAPI staining and CARD-FISH) of a M. aeruginosa bloom sample.
M. aeruginosa cells appear pink or pinkish blue on account of chlorophyll autofluorescence. Blue signals are from bacteria negative for the BAG645 probe. Spotted positive signals (yellow-green) of the BAG645 probe are observed mostly from bacteria associated with the colony and cells of M. aeruginosa, not from free-living bacteria. Note that two morphological types of M. aeruginosa, differing in cell size, are found in the sample and that the FISH signals are more intense in the right-hand colony.