Figure 7
The CD titration isotherm of 10 μM MACP6 with the addition of c-kitG20T G-quadruplex in 17 mM phosphate buffer solution (5% DMSO) at 20 °C (⚬ or • indicate the change at 380 nm or 345 nm in CD spectra of MACP6, respectively). The CD spectra values from 320 nm to 430 nm were used for the calculation of Ka1 ((9.4 ± 0.4) × 104 M−1) and Ka2 ((1.1 ± 0.07) × 105 M−1) using the Hyperquad 2003 program51. The red lines show the least-square nonlinear fitting of the experimental data to the appropriate equations. The wine dot, green dash and pink dot dash lines show the calculated percentage of compound species including [MACP6], [MACP6•c-kitG20T] and [MACP6•(c-kitG20T)2] vs. the concentration of MACP6 at each additional c-kitG20T concentration.
