Figure 2
From: Exercise intolerance and developmental delay associated with a novel mitochondrial ND5 mutation
Respiratory complex assembly and in-gel activity assay.
(A) Whole cells were solubilized with n-dodecyl-β-D-maltoside (DDM) and then subjected to BN-PAGE/immunoblot analysis. Complexes I, II, III, IV and V were immunoblotted with anti-Grim19, SDHA, core2, COX IV and ATP-5A antibodies, respectively; β-VDAC was used as internal control. (B) Mitochondria were solubilized with DDM. In-gel activity assays of complexes I and IV were performed following BN-PAGE analysis; the VDAC protein was used as a loading control. All BN-PAGEs were run under the same conditions (5 mA per gel for 1 h and followed by electrophoresis at 200 V for 2 h). Full-length blots are presented in Supplementary Figs. 1 and 2. Error bars, ±SD *, P < 0.05.
