Figure 3

Respiration assay of cells containing the m.12955A>G mutation.

(A) Mitochondrial respiratory capacities were determined in clone #9, #17 and #18 cells. Oligomycin (2.5 μg/ml) was added for the measurement of uncoupled mitochondrial respiration. OXPHOS coupling respiration was calculated by subtracting the uncoupled component value from the total endogenous respiration value. (B) Respiratory coupling efficiencies were presented as the ratio of basal oxygen consumption to the oxygen consumption with oligomycin. Error bars, ±SD. *, P < 0.05;