Figure 8
From: VRK1 regulates Cajal body dynamics and protects coilin from proteasomal degradation in cell cycle
Nuclear export is necessary for degradation of ubiquitinated-coilin.
A MCF7 cells, either control or in which VRK1 was knocked-down, were treated with leptomycin B and its effect on the presence of coilin in nuclei determined by immunofluorescence confocal microscopy. B Accumulation of ubiquitinated coilin in nuclei in cells treated with leptomycin B. C Effect of MG132 or leptomycin B on the level of nuclear coilin in cells in which VRK1 was knocked-down. D Effect of leptomycin B on coilin nuclear levels after serum deprivation or after VRK1 knock-down.
