Table 1 Primers used in Qrt-PCR.

From: Incubation temperature affects the immune function of hatchling soft-shelled turtles, Pelodiscus sinensis

Gene

Genebank accession number

Primers

IgD

FJ605149

F: 5′ - TGGGAACAAGGCACCAGATTT -3′

  

R: 5′ - TTCGCAGACAAGAGTCAAGGA -3′

IgM

FJ605150

F: 5′ - GCTTATCCCACCGACCTTTG -3′

  

R: 5′ - TCATCTCCTCGCTCCCACTC -3′

CD3γ

GU168571

F: 5′ - ATGAGGAGGGCGAGCACC - 3′

  

R: 5′ - GCAAACGCATTACAAGGAGGA - 3′

CD9

FJ975143

F: 5′ - TATCCTCTGCGTCCCGTCC - 3′

  

R: 5′ - CAAACCGAAGCCATAGTCCAA - 3′

β-actin

EU727174

F: 5′ - TGTTACCCATACTGTGCCCATC - 3′

  

R: 5′ - TAGCCATCTCCTGTTCAAAATCC -3′

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