Figure 1
Design, expression and purification of full-length VP1 and truncated VP1 proteins. (a) The design of the five truncated VP1 proteins. (b) Expression and purification of recombinant full-length EV71 capsid proteins, VP0, VP1 and VP3 and the five truncated VP1 proteins. The expression of the recombinant full-length EV71 capsid proteins and truncated VP1 proteins was induced with IPTG and the relative molecular weights (MW) of the expressed products were 52,898 for VP0, 44,151 for VP3, 50,371 for VP1, 46,230 for VP141-297, 44,361 for VP161-297 36,111 for VP1134-297 and 23,730 for VP11-60, 19,002 for VP145-58. The expressed products were purified using a Ni-NTA column affinity chromatography.
