Figure 1
miR-26a is an antagonist of PRRSV.
(A) MARC-145 cells were transfected with plasmids expressing miR-Ctr, miR-323, miR-654, miR-591, miR-608, miR-601, miR-26a, miR-509, miR-378, miR-939 and miR-491 or vector control (1 μg each). At 24 hpt, the cells were infected with VR2332 PRRSV at 0.1 MOI. The viral titers of the supernatants at 72 hpi were determined as the TCID50. (B) MARC-145 cells were infected with or without the PRRSV VR-2332 strain at 1 MOI for the indicated time. The expression of miR-26a was measured by q-PCR and normalized to the expression of miR-18 in each sample. (C) MARC-145 cells were infected with or without the PRRSV VR-2332 strain at the indicated MOIs for 36 h and miR-26a expression was measured as described in (B). These data are expressed as the mean ± SD and compared to the “virus only” (A) or “0 hpi” (B and C) groups by two-way ANOVA; * P < 0.5, ** P < 0.01 and *** P < 0.001.
