Figure 2
miR-26a is a broad-range antagonist of viral replication.
MARC-145 cells were transfected with plasmids encoding miR-26a or control (miR-Ctr). Twenty-four hours post-transfection, the cells were infected with H1N1 and H9N2 IAV (A), VSV (B), HSV-1 (C), VR2332 and JXwn06 PRRSV (D) at a MOI = 1 for 72 h. The viral titers were determined as the TCID50. (E) The relative expression of miR-26a in MARC-145 cells transfected with miR-26a or miR-Ctr, as well as in mock-transfected cells, was measured by real-time PCR at the indicated times. (F) The viability of MARC-145 cells mock-transfected or transfected with miR-26a or miR-Ctr was determined by MTT assays up to 120 hpt. These data are expressed as the mean ± SD and compared to the miR-Ctr group (A-D) or mock group (E-F) by paired-sampled t-tests and two-way ANOVA; * P < 0.5, ** P < 0.01 and *** P < 0.001.
