Figure 5

Cca-specific Th2 response converts intra-Cca Tregs to Th9 cells.

CD4⁺ CD25⁺ CD127⁺ T cells and DCs were isolated from mice sensitized to Cca-e or U87-e. Intra-Cca Tregs were isolated from Cca-tissue and labeled with CFSE. CD4⁺ T cells and Tregs were cultured at a ratio of 1:1 in the presence of DCs, Cca-e (or U87-e, or BSA) (10 μg/ml) and IL-2 (10 ng/ml) for 10 days. The additional treatment is denoted above each subpanel. The cells were collected and stained with an anti-IL-9 antibody and analyzed by flow cytometry. A, the gated dot plots indicate CFSE⁺ cells. B–H, the histograms indicate the frequency of IL-9⁺ cells in the gated cells of A. I, the bars indicate the summarized data of B–H. The data of bars are presented as mean ± SD. *, p < 0.01, compared with group B. Each group consists of 10 mice. The data are representatives of 10 independent experiments.