Figure 7
Out of 241 GPCRs, JWH133 only elicits β-arrestin recruitment at CB1 and CB2.
JWH133 (10 μM) was tested for its ability to induce β-arrestin recruitment at 241 GPCRs, split over two panels, using the Discoverx PathHunter® eXpress β-Arrestin GPCR Assay system. (A) The GPCRMax panel was comprised of GPCRs with known and validated ligands. For this panel % Agonist activity is calculated as 100% x (mean of test sample - mean of vehicle control) / (mean MAX control ligand - Mean of vehicle control). JWH133 only caused β-arrestin recruitment at CB1 and CB2. (B) The OrphanMax panel was comprised of GPCRs without validated ligands. For this panel % Agonist activity is calculated as 100% x (mean of test sample - mean of vehicle control) / (mean of vehicle control). JWH133 did not elicit β-arrestin recruitment at either GPR18 or GPR55. Data are mean of technical duplicates.
