Figure 5
Gene expression analysis of N. crassagrown in a CSTR with periodic light/dark cycles.
N. crassa was cultured in Vogel’s medium containing 1% (w/v) glucose and 0.08% (v/v) polyacrylic acid (MW ~100,000) at 25 °C. Reactors were exposed to light for 24 hrs following inoculation then exposed to cycles of darkness (11 hrs) then light (11 hrs) for the duration of data collection. The shaded region corresponds to dark periods. Gene expression of (a) frequency (frq), (b) white collar-1 (wc-1), (c) white collar-2 (wc-2), (d) vivid (vvd) and clock-controlled genes (e) ccg-1, (f) ccg-4 and (g) ccg-14 were measured over time and were analyzed using the ΔΔCT method with btl and vma2 as reference genes. Shown are the data collected from one of three biological replicates and error bars represent SD between triplicate samples within one biological experiment.
