Table 1 Knock-in efficiency with various combinations.
From: CRISPR/Cas9-mediated reporter knock-in in mouse haploid embryonic stem cells
Combination No. | Cas9 type | sgRNA | HA of donor vector | The Venus-positive ratios | ||
---|---|---|---|---|---|---|
 |  | target | targeting seq. (nt) |  | Haploid (%) | Diploid (%) |
1 | Cas9 | Actb | R(20) | Actb | 18.0±1.2 | 8.2±0.6 |
2 | Cas9 | Actb | R(17) | Actb | 21.2±0.9 | 10.3±0.3 |
3 | Cas9n | Actb | L(20)+R(20) | Actb | 30.6±1.5 | 14.3±0.4 |
4 | Cas9n | Actb | L(20)+R(17) | Actb | 31.1±1.3 | 14.4±0.6 |
5 | Cas9n | Actb | R(20) | Actb | 11.4±1.4 | 8.8±0.4 |
6 | Cas9n | Actb | R(17) | Actb | 4.0±0.5 | 3.3±0.2 |
7 | Cas9n | Sox1 | L(20)+R(17) | Actb | 0.0±0.0 | 0.1±0.1 |
8 | Cas9n | Actb | L(20)+R(17) | Sox1 | 0.1±0.1 | 0.0±0.0 |
9 | No |  |  | Actb | 0.0±0.0 | 0.0±0.0 |
 | The ratio of knock-in ESC lines | |||||
Haploid (%) | ||||||
10 | Cas9n | Sox1 | L(20)+R(17) | Sox1 | 46.2 (n=26) | Â |