Figure 1 | Scientific Reports

Figure 1

From: Automated Pipeline for Purification, Biophysical and X-Ray Analysis of Biomacromolecular Solutions

Figure 1

SEC-SAXS/TDA set-up at the EMBL-P12 beamline.

(a) Using a micro-splitter valve, the eluting stream is divided in equal parts enabling in-parallel SAXS and light-scattering measurements.(b) Automated SEC-SAXS pipeline trace of I(0) vs. frame number. Bovine serum albumin was analyzed without TDA detectors (SEC-SAXS; ◯), with a parallel SEC-SAXS/TDA split stream set-up (●) and in-line SEC-TDA-SAXS set-up (△). *, ** indicate the peaks corresponding to the monomeric and dimeric fraction, respectively. (c) Schematic of the P12 data processing pipeline with integrated SEC-SAXS/TDA modules.

Back to article page