Figure 4
Kinetic analysis of PVSN-dependent inhibition of PVSN using FAM-pStat1 and RP-UFLC.
(A): Plots of the natural logarithm of the percentage residual PTPN2 activity versus time for each PVSN concentration. The apparent first-order inhibition constants (kobs) for the different concentration of PVSN were determined from the slopes of linear regression lines. (B): ki determination. The kobs were plotted against PVSN concentrations and the second-order inhibition constant (ki) was determined from the slope of the linear regression lines.
