Figure 3
From: Insecticide resistance in the bed bug comes with a cost

Experimental design for selection experiment. Bugs were exposed to label rate Temprid SC® for a time calculated to kill 80% of the respective strain of bed bugs22. Two parental lines of selected and unselected bugs were created for each strain. Using the subsequent F2 generation from these lines that receive no further exposure to insecticide, two samples were taken from each replicate to generate data. Replicates were not synchronous and separated by weeks.