Figure 6

ACH-11 inhibited arterio-venous shunt thrombosis in rats in vivo.

After the administration of ACH-11 (0.33, 1 and 3 mg/kg), aspirin and the vehicle, rats were anesthetized by intraperitoneal injection of chloral hydrate (350 mg/kg) and an arterio-venous shunt tube (12 cm, containing 10-cm long of 4 braided silk thread) was installed between the right carotid artery and left jugular vein of each rat. After 20 min, the silk thread was removed from the shunt tube and its wet weight was immediately measured. The dry weight of the thread was measured 6 h later at room temperature. The wet and dry weights of the formed thrombi were determined by subtracting the pre-experiment weights of the wet and dry 6-cm threads, respectively. Data are the mean ± SD, ^*P < 0.05, ^***P < 0.001 and ^****P < 0.0001 versus vehicle, n = 6, analyzed by one-way ANOVA, followed by the Tukey multiple comparison test.