Figure 3 | Scientific Reports

Figure 3

From: A repeat unit of Vibrio diarrheal T3S effector subverts cytoskeletal actin homeostasis via binding to interstrand region of actin filaments

Figure 3

F-actin protection from depolymerization by VopVrep1.

(a) F-actin stabilization was examined using a high-speed cosedimentation assay. A mixture of depolymerizing F-actin in the absence or presence of VopVrep1, latrunculin B and phalloidin was ultracentrifuged after 0, 2, or 4 h incubation. The pellet fractions (F-actin) were separated using SDS-PAGE and the gels were stained with Coomassie blue. (b) The stabilized F-actin shown in (a) was analyzed using densitometry. The relative F-actin ratio was calculated as the amount of F-actin pelleted at each time point divided by that of zero time point amount.

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