Figure 2
From: The HAB1 PP2C is inhibited by ABA-dependent PYL10 interaction
BSA can enhance PP2C phosphatase activity.
Inhibition of PP2C (HAB1172-511) phosphatase activity by PYL10 (A), PYL2-wt (B) and PYL2-I88K (C) at various concentrations of BSA (0, 20, 50, 80, 100, 150, 300 μg/ml) in the presence or absence of ABA at different ratios of receptor to PP2C (10:1, 2:1). Isothermal titration calorimetry (ITC) analysis of PYL10 with different concentration of BSA (D) or ABA (E). Dissociation constants were calculated from the ITC data; Kd = 3.00 ± 0.27 μM (D) for BSA/PYL10 and Kd = 66.22 ± 13.86 μM (E) for ABA/PYL10. The relative large energy variation of BSA injection relative to ABA injection probably due to the large size of BSA and non-specific binding of BSA to host proteins.
