Table 2 LCN2 detection in serum. Eight samples from 4 HCC patients and 4 healthy individuals were directly applied to the aptamer-based assay well and then incubated with reporter aptamer. For comparison with the ELISA kit performance, serum samples were 30-fold serial diluted and experiments were performed according to the manufacturer’s instructions. Statistical analyses were performed using the R Foundation statistical computing software. Statistical values were determined by Student’s t-test. Results are expressed as the mean ± SEM. P > 0.05 indicates that there was no significant difference in the analytic comparison between the ELISA kit and aptamer-based assay performance.
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