Figure 2

Renal injury in RIAKI.

Mice were sacrificed at the indicated time points after 5 mL/kg glycerol (Gly) or vehicle (Veh) administration. (a) Representative images of PAS staining in the cortex and outer medulla (OM) are shown (black arrowheads, lytic lesions of the tubular epithelium; white arrowheads, intratubular casts). (b) The tubular injury score in the cortex and OM was determined (n = 3 for each). (c) Serum levels of BUN and Cr were assessed (n = 3–5 for each). (d) Renal mRNA levels of Havcr1 and Lcn2 were assessed by real-time RT-PCR analysis (n = 3–5 for each). Data are expressed as mean ± SEM. *p < 0.05, **p < 0.01.