Figure 3 | Scientific Reports

Figure 3

From: Extracellular Matrix can Recover the Downregulation of Adhesion Molecules after Cell Detachment and Enhance Endothelial Cell Engraftment

Figure 3

Apoptosis and angiogenesis analysis of early stage after hESC-ECs transplantation.

(a) Representative photomicrographs showing TUNEL staining in the injected cell clumps at day 3. Cell apoptosis of hESC-EC transplantation (up panel) and hESC-ECs transplanted with Matrigel (hESC-EC + M; lower panel). Green channel shows GFP-labeled graft cells. Red channel shows TUNEL fluorescent staining. Nuclear staining is identified by DAPI (blue). hESC-EC (n = 5), hESC-EC + M (n = 5). (b) Quantitative analysis of TUNEL assay. hESC-EC apoptosis were inhibited by Matrigel application. (c) Confirmation of hESC-EC engraftment by immunostaining. Clump formation of injected hESC-ECs was observed and immunostaining with GFP (green) and α-sarcomeric actin (α-SA) (Cyan) at day 3. Immunostaining with mouse CD31 (red) revealed more host vasculatures extended into injected cells clumps (Note: mouse CD31 antibody not react with hESC-EC). (d) Quantitative analysis revealed increased microvascular density (MVD) per high-power field (HPF) by Matrigel application. The lines indicate the graft region defined by the GFP-labeled graft cells. Nuclear staining is identified by DAPI (blue). *P < 0.05 compared to control. Scale bar = 50 μm. hESC-EC (n = 5), hESC-EC-M (n = 5). hESC-EC + M, hESC-ECs transplanted with Matrigel.

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