Figure 2 | Scientific Reports

Figure 2

From: Molecular and structural analysis of Legionella DotI gives insights into an inner membrane complex essential for type IV secretion

Figure 2

DotI and DotJ form a stable complex.

(A) BN-PAGE analysis of L. pneumophila lysates from wild-type and deletion strains. Deleted genes are indicated above the Western immunoblot using DotI antibody. (B) Two-dimensional PAGE analysis of L. pneumophila producing M45-DotJ. Samples were separated by BN-PAGE and then gel slices were subjected to second-dimensional SDS-PAGE. Western images of aligned one (top) and two (bottom) -dimensional PAGEs using DotI and M45 antibodies are presented with positions of molecular weight markers (in kDa). (C) Co-immunoprecipitation of DotI and M45-DotJ. Lysates from L. pneumophila producing M45-DotJ were subjected by immunoprecipitation with or without M45 antibody, followed by Western immunoblotting analysis using DotI or M45 antibodies.

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