Figure 1

Study of polymerase read-through of single triazole junction.
A) Scheme for substrate synthesis and read-through study. B) LC trace of control read-through experiment using Klenow with wild-type template. UV detection at 495ānm. C) LC trace of read-through products using Superscript IIITM. D) LC trace of read-through using Klenow fragment. E) Mass spectrum of major peak in trace D).