Figure 2 | Scientific Reports

Figure 2

From: Electric Pulse Stimulation of Myotubes as an In Vitro Exercise Model: Cell-Mediated and Non-Cell-Mediated Effects

Figure 2

EPS conditioned medium alters gene expression in FAO hepatocytes and primary rat hepatocytes.

A: Affymetrix microarray analysis of FAO hepatocytes stimulated with C2C12 conditioned media (CM). Four individual experiments were performed and the genes significantly (p < 0.05) up- and down-regulated (up, FC > 1.15; down, FC < −1.15) after both 2 h and 16 h stimulation are depicted. Inductions shown are compared to 2 hour and 16 hour stimulation with CM without EPS. Color scale ranges from a signal log ratio of −2 (green) to 5 (red). B: Relative gene expression of Mt1, Mt2, Slc30a2 and Cyp2a3 in FAO hepatocytes measured by RT-PCR. C: Relative gene expression of Mt1, Mt2, Slc30a2 and Cyp2a3 in primary rat hepatocytes measured by RT-PCR. (B-C) Error bars represent SD values of biological triplicates. * = p < 0.05; ** = p < 0.01; *** = p < 0.001 compared to CM without EPS at the same time point.

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