Figure 4 | Scientific Reports

Figure 4

From: Electric Pulse Stimulation of Myotubes as an In Vitro Exercise Model: Cell-Mediated and Non-Cell-Mediated Effects

Figure 4

EPS can cause cell-independent changes in cell culture media that can subsequently affect hepatic gene expression.

A: Experimental set-up, stimulation of FAO hepatocytes with EPS-CM generated with and without C2C12 cells. B: Relative gene expression of Mt1, Mt2, Slc30a2 and Cyp2a3 after 2 hour treatment was measured by RT-PCR. C: Relative gene expression of Mt1, Mt2, Slc30a2 and Cyp2a3 after 16 hour treatment was measured by RT-PCR. Error bars represent SD values of biological triplicates. ** = p < 0.01; *** = p < 0.001 compared to CM without EPS.

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