Figure 2 | Scientific Reports

Figure 2

From: Noninvasive imaging of sialyltransferase activity in living cells by chemoselective recognition

Figure 2

FRET responses of TRITC-proteins after incubation with FITC-APBA.

(a) 12 nM FITC-APBA and (b) TRITC-fetuin at a TRITC amount of 160 nM after incubation with 0, 4, 8, 12 and 16 nM FITC-APBA (from bottom to top) for 30 min under donor excitation. (c) Plots of FI change (Δ%) at 572 nm vs FITC-APBA concentration. Δ%=[I/IC − 1]×100%, where I and IC are the FI of TRITC-proteins in the presence and absence of FITC-APBA, respectively.

Back to article page