Figure 4
The deletion of 90–188 amino acid of JWA played a major function for topoisomerase IIα degradation.
(a) Schematic showed the domains of full length and indicated fragments JWA protein. “Dark grey” represented the TMD of JWA. Amino acid sequences ranged from 1 to 60 for JWA-1, 1 to 90 for JWA-2, 1 to 140 for JWA-3 and 141–188 for JWA-4. (b) NCI-H460 cells were transiently transfected with full-length Flag-JWA plasmid and four different fragments of JWA plasmid respectively. Whole-cell extracts were prepared 24 h after transfection and topoisomerase IIα expression was measured by western blotting. Error bars represent the mean ± SD of triplicate experiments. Statistical differences to the controls were shown as *p < 0.05, **p < 0.01.
