Figure 5
Cell cycle modulated the inverse regulation of topoisomerase IIα and JWA protein expression.
NCI-H460 cells were plated on 60 mm plates in DMEM (10% fetal bovine serum), which was changed to starving DMEM (without serum) for 24 h or 30 h. (a) Flow cytometric analysed the change of cell cycle. And the protein levels of JWA and topoisomerase IIα were examined by Western blotting. (c) and (d) Then NCI-H460 cells were treated with 200 nM nocodazole, respectively for 12 h, 18 h and 24 h. (b) Flow cytometric analysed the cell cycle. (e) and (f)The expression of JWA and topoisomerase IIα were examined by Western blotting. β-actin expression served as a loading control. Error bars represent the mean ± SD of triplicate experiments. Statistical differences to the controls were shown as *p < 0.05, **p < 0.01 and ***p < 0.001.
