Figure 2
From: Membrane-less microfiltration using inertial microfluidics
(a) Sample processing workflow showing process of cell enrichment using the high throughput filtration system from spinner flasks imitating condition of a perfusion bioreactor. Cell cultures are subject to inertial filtration system and used media are assessed for product e.g., IgG concentration. Fresh media and enriched cell samples are added back to the flask following each filtration process. (b) Separation efficiency of CHO cells as a function of concentration at 6 mL/min flow rate for a single spiral device. (c) Viable cell density and viability of hybridoma cells for 10 days continuous culture. The results indicated that both parameters are not significantly affected by inertial filtration. (d) Rate of IgG production by hybridoma cells over 10 days. A steady increase in IgG was observed, suggesting that cellular activities involved in IgG production were minimally affected by inertial filtration (e) Evaluation of stress levels of processed cells compared to cells incubated at 37 °C in cell culture media, or at 24 °C in the PBS buffer. Up-regulation of c-FOS gene was evident in response to cycloheximide (CHX) treatment38 which was considered a positive control. The gene expression was normalized to the GAPDH housekeeping gene for CHO cells for different conditions.
