Figure 4
From: The Tie2-agonist Vasculotide rescues mice from influenza virus infection
VT has no antiviral activity and does not affect innate immunity.
(a) VT (in vitro 20 ng/mL, in vivo 500 ng) had no effect on viral replication assessed by qPCR in vitro or using lung homogenates for plaque-forming units (pfu) on day 5 post-infection. (b–c) Chemotaxis of THP1 monocyte-like cells or human neutrophils was not affected by VT, ***p < 0.001. (d–e) Significant effect of VT on oxygen saturation and weight loss of mice prior to histological analysis, **p < 0.01. (f) Influenza induces alveolar neutrophilia (day 5 post-infection) that is unaffected by VT, *p < 0.05. (g) Systematic scoring of acute lung injury by histology was performed in a blinded fashion. Data are mean and standard deviation from n = 5 mice in Flu and Flu/VT48 groups and n = 3 uninfected controls.
