Figure 2 | Scientific Reports

Figure 2

From: A Sweet Spot for Molecular Diagnostics: Coupling Isothermal Amplification and Strand Exchange Circuits to Glucometers

Figure 2

Sensitive and specific detection of synthetic MERS-CoV DNA (sORF1A) using LAMP-to-glucose transduction.

The bar-graph presents glucometer responses to both non-target (RPOB) and different amounts of target (MERS-CoV sORF1A = MERS) templates. N-1, N-2, P-1 to P-4 were amplification reactions with the ORF1A.55 MERS-CoV primer set. Cognate targets (P) respond while non-cognate targets or negative controls (N) do not. Assays included a 1.5 hour 55 oC LAMP reaction, 1 hour 25 oC OSD and 40 min 55 oC glucose generation using a commercial yeast invertase (yeast INV). The error bars represent standard deviations calculated from three parallel assays.

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