Figure 4 | Scientific Reports

Figure 4

From: A Sweet Spot for Molecular Diagnostics: Coupling Isothermal Amplification and Strand Exchange Circuits to Glucometers

Figure 4

Stability and reproducibility of modified magnetic beads.

(A) LAMP-to-glucose assays with 2E4 copies of MERS-CoV sORF1A target DNA (Day 1, Day 2 and Day 3, red) and buffer controls (Day 1, Day 2 and Day 3, green), carried out with conjugated magnetic beads (Inv-FPc/FP/MBs) that were prepared on three different days. On each day, three parallel assays were carried out with both the target MERS-CoV sORF1A DNA and buffer controls. A total of nine assays were performed for both the sORF1A target and buffer controls. Assay conditions were otherwise the same as in Fig 3. (B) Time dependence of glucometer responses to LAMP amplicons generated from 2E4 copies of sORF1A using a single preparation of Inv-FPc/FP/MBs. The error bars represent standard deviations calculated from three parallel assays. The commercial yeast INV was used in these experiments.

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