Figure 5 | Scientific Reports

Figure 5

From: A Sweet Spot for Molecular Diagnostics: Coupling Isothermal Amplification and Strand Exchange Circuits to Glucometers

Figure 5

Detection of synthetic ZEBOV DNA (ZEBOV-VP30) using LAMP-to-glucose transduction.

Glucometer responses to 1.5E2 copies (P-1), 1.5E3 copies (P-2) and 1.5E4 copies (P-3) of synthetic ZEBOV VP30 (= ZEBOV) or to a DNA buffer control (0 copies, N-1). N-2 is the negative control for OSD signal transduction reaction and shows no response even in the presence of an amplification reaction seeded with 2E4 copies of the non-cognate MERS-CoV sORF1A (= MERS) template and appropriate MERS-specific primers. Reaction conditions were as in Fig 3. The error bars represent standard deviations calculated from two parallel assays. The thermostable TmINV was used in these experiments.

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