Figure 6

Using an OR gate to improve the robustness of sensing.
(A) OR gate design. Either the ORF1A region or the upE region of amplicons produced from a MERS-CoV template could initiate OSD. The respective amplicons will displace either an Inv-ORF1A-Fc or an Inv-upE-FPc specific reporter strand bound to the complementary OR-P capture strand attached to magnetic beads. Either or both released invertases will lead to a glucometer response. (B) Performance of the OR gate with RNA extracted from 60 PFU MERS-CoV virons amplified with either no primers, with the upE.9 and ORF1A.55 individually, or with a combination of the upE.9 and ORF1A.55 primers. Reaction conditions were as in Fig 3. The error bars represent standard deviations calculated from two parallel assays. The thermostable TmINV was used in these experiments.