Table 1 Sequences of oligonucleotides used in this work (5’ to 3’)
ORF1A.55 primer set | F3a | TTATGCAAACATAGTCTACGAG |
B3 | CGCAAAGTTAGAAAGTGATGG | |
FIP | AAGCATTAGTGGGGGCAAGCCCCACTACTCCCATTTCG | |
BIP | ATGCGCACTACACATACTGATATTTGTACAATCTCTTCACTACAATGA | |
LP | GGTGTCTACATTAGTATGTCACTTGTATTAG | |
sORF1AOSD probe set | FP | CGAAGCCAATTTGCAACTGCAATCAGCGCTGAG AAAAAAAAA /3’Biotin/ |
FPc | ATTGCAGTTGCAAATTGGCTTCG AAAAAAAAAAAA /3’Thiol/ | |
upE.9primer set | F3 | AGTAAGATTAGCCTAGTTTCTGT |
B3 | TCCATATGTCCAAAGAGAGAC | |
FIP | GAGGAACTGAATCGCGCGTTGACTTCTCCTTAAACGGCA | |
BIP | TTCACATAATCGCCCCGAGCTAATGGATTAGCCTCTACACG | |
LP | GCAGGCACGAAAACAGTGGAAACAT | |
ORGateOSD probe set | ORP | /5Biosg/AAAAAAAAAATCGCTTATCGTTTAAGCAGCTCTGCGCTAC TATGG GTCC CGAAGCCAATTTGCAACTGCAATCAGCGCTGAGC |
1A-FPc | /5ThioMC6-D/ AAAAAAAAAAAA ATTGCAGTTGCAAATTGGCTTCG | |
upE-FPc | /5ThioMC6-D/AAAAAAAAAAAA TAGCGCAGAGCTGCTTAAACGATAAGCGA | |
Mimical target | 1A-T | CTCAGCGCTGATTGCAGTTGCAAATTGGCTTCG |
upE-T | GGACCCATAGTAGCGCAGAGCTGCTTAAACGATAAGCGA | |
Random sequence (or Random C) | CGACATCTAACCTAGCTCACTGAC’ | |
ZEBOV. 3.3 primer set | F3 | AGACAGCATTCAAGGGATG |
B3 | CCTTTTTTCAAGGTCGGACA | |
FIP | CTCCTTGATTGACGGTACTCACCGACACGACCACCATGTTC | |
BIP | CTCACAAGTGCGCGTTCCTAATGTCTTTAGGTGCTGGAG | |
ZEBOV OSD probe set | ZEBOV-FP | GAGCACGATCATCATCCAGAGAGAATTATCGAGGTGCA AAAAAAAAAA/3’Biotin/ |
ZEBOV-FPc | TGCACCTCGATAATTCTCTCTGGATGAT AAAAAAAAAAAA /3’Thiol/ |
