Figure 2
From: Cell proliferation by silk gut incorporating FGF-2 protein microcrystals
Encapsulation of FGF-2 within polyhedra in the posterior silk glands of transgenic silkworms.
(A) Detection of the recombinant protein in posterior silk glands. Protein samples (7 μl) from w1-pnd strain (lane 1), BmFibH-polyhedrin line (lane 2), BmFibH-H1/FGF-2 line (lane 4) and BmFibH-polyhedrin/H1/FGF-2 line (lane 5) posterior silk glands on the 6th day of the 5th instar were electrophoresed in a 12.5% gel with a molecular marker (lane M), rhFGF-2 (lane 3) and polyhedron-encapsulated H1/FGF-2 prepared from cultured Sf21 cells (lane 6); FGF-2 (upper panel) and polyhedrin (lower panel) were detected by immunoblotting using anti-polyhedrin and anti-FGF-2 antibodies as described in the Methods section. Degraded H1/FGF-2 bands (approximately 17 kDa) were observed in lane 6 (upper panel). Arrows indicate polyhedrin, H1/FGF-2 and rhFGF-2. Original gel images of the data are presented in Supplementary Figure S2. (B) Polyhedra formed in the posterior silk glands of BmFibH-polyhedrin and BmFibH-polyhedrin/H1/FGF-2 lines were fixed on a glass-based dish and the H1/FGF-2 encapsulated within the polyhedra was detected by immunofluorescence as described in the Methods section. Bar, 50 μm.
