Figure 4
From: Cell proliferation by silk gut incorporating FGF-2 protein microcrystals
FGF-2 activity changes after incubation for one week at 25 °C.
Silk gut powder samples (1 mg) from the BmFibH-polyhedrin/H1/FGF-2 and BmFibH-H1/FGF-2 lines suspended in PBS and diluted rhFGF-2 (10 ng) were placed on the inside filters of cell culture inserts. The samples in the cell culture inserts were air-dried and kept for one week at 25 °C (treated samples; column T). Untreated (column U) and treated samples in cell culture inserts were introduced into culture of serum-starved NIH3T3 cells. After 48 h of cultivation, cell counts were measured by WST-8 assay. Mean relative values of triplicate experiments are presented with standard deviations. *P < 0.01 vs. cells cultured with each sample of rhFGF-2 and silk gut powder from the BmFibH-H1/FGF-2 line after incubation for one week at 25 °C.
