Figure 5 | Scientific Reports

Figure 5

From: Biorealistic cardiac cell culture platforms with integrated monitoring of extracellular action potentials

Figure 5

Reusability of micro-engineered MEAs.

(a) Electrode impedance measurements from a brand-new, non-processed MEA (blue, n = 56 electrodes), from the same MEA after processing (red, n = 49 electrodes) and after the first use with cells (green, n = 56 electrodes). Immunofluorescence images of NRVM cultured on (b) initial and (c) reused micro- engineered MEAs, stained for plasma membrane (green-wheatgerm agglutinin) and nuclear DNA (blue-DAPI). Scale bars, 50 μm. (d) Quantification of cellular alignment. Error bars indicate standard error of mean. n represents the number of cells. *** indicate P < 0.001. (e) Comparison of conduction velocity profiles of NRVM on an initial (left column) and reused (right column) MEA.

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