Figure 4

PALM imaging.

PALM imaging of mitochondria (mito-Dendra2 in HEK293 cells) analyzed by SNSMIL: In 4a–4d the super-resolution images generated with SNSMIL under different parameter settings are shown. In 4e the corresponding TIRF image is depicted. An enlarged view of the boxed region is shown below each image. Fitting model, value and number of emitters reconstructed for the whole and the zoomed images are listed below: (a) Model 1; 2.0; 3,270,739; 245,259 (b) Model 1; 1.0; 3,785,947; 262,522 (c) Model 1; 4.0; 947,779; 104,470 (d) Model 2; 2.0; 1,825,069; 132,350 (PWT is fixed as 3.0). A selected finger-like structure is marked by a yellow box (4a, lower part) and projected onto the x-axis marked as yellow line. (f) Line profiles of a straight mitochondrial structure were plotted for images 4a–4e. Each line profile through a reconstructed SMLM image is normalized to its maximal intensity. Intensities from the line profile through the same structure in the corresponding TIRF image are normalized to match the same scale. Scale bar is 4 μm.