Figure 1
From: DW-F5: A novel formulation against malignant melanoma from Wrightia tinctoria
DW-F5 does not induce pharmacological toxicity.
(a,b) DW-F5 induces significant cytotoxicity in the melanoma cell line, A375, while normal skin fibroblast (FS) and epithelial cells (HEMa-LP) are unaffected as determined by microscopic observation and MTT assay. (c,d) H&E stained liver tissues of mice treated with or without DW-F5 for a period of 7 days and 3 months. (e,f) The serum level of total proteins, albumin and globulin in DW-F5 treated mice for a period of 7 days and 3 months is represented graphically. (g,h) Graphical representation of the serum level of total and direct bilirubin in DW-F5 treated mice for a period of 7 days and 3 months. (i,j) The activity of toxicological markers such as serum alkaline phosphatase (ALP), serum glutamic oxaloacetic transaminase (SGOT) and Serum glutamic pyruvic transaminase (SGPT) in DW-F5 treated mice for a period of 7 days and 3 months is graphically represented. Though there was a statistically significant deviation in the values of some toxicological parameters between the control and treated groups, the varied values were within the normal range. Data represent three independent sets of experiments. The error bars represent ± S.D. ANOVA (for MTT assay) or Student’s t test (for toxicological studies) was used for statistical comparison among different groups. * P ≤ 0.05; ** P ≤ 0.01; # P ≤ 0.001, ns non significant.
